VIJIMICROBES

  DEPARTMENT OF MICROBIOLOGY BIOINSTRUMENTATION – 23UMBE21 Course Outcomes: CO1 Relate the facts, ideas and need of equipment’s in the field of molecular analysis (K1) CO2 Explain the theoretical skills behind the usage of biomedical instruments (K2) CO3 Understand the basic principles and types of analytical techniques in medical diagnosis (K2) CO4 Apply the knowledge about the applications of radioactivity and its measurements in biomolecules identification, separation as well as imaging techniques (K3) CO5 Compare the efficacy and make use of modern techniques to rectify the problem in an efficient way (K3) UNIT I Basic instruments: pH meter, Centrifuge- Preparative, Analytical and Ultra, Laminar Air Flow, Autoclave, Hot Air Oven and Incubator. Buffers- Phosphate, Acetate, TE, TAE. Biological importance of buffers. (12 Hours) UNIT II Spectroscopic Techniques: Colorimeter, Ultraviolet and visible, Infrared and Mass Spectroscopy. (12 Hours) UNIT III Chromatographic and Electrophoresis